While many variables might influence an individual’s willingness to express their faith in the workplace, the role of regional context has not been fully considered. The different geographical regions in the U.S. consist of unique demographics and cultures that could shape an individual’s expression of faith at work. Moreover, these regional effects might be moderated by an individual’s specific religious tradition. Using data from a survey of U.S. adults featuring oversamples of Jewish and Muslim individuals, we utilize two unique measures of religious expression—displaying/wearing religious items at work and talking about religion at work—to assess the roles of region and religious tradition in expression of faith at work. We find that regional cultures can sometimes override religious subcultures to determine if and how people express their religion in the workplace. We find that evangelical-conservative Christians are more likely than those following most other religious traditions to say that they talk about their faith at work, regardless of the region in which they reside. However, we also find that individuals in the South tend to be more likely to express their faith in the workplace independent of their religious tradition while evangelicals in the Northwest are less so. The findings have broader implications for subcultures related to religious pluralism in an increasingly diverse U.S. society.